ASTM D – 15 Standard Test Method for Analysis of Aqueous Extractable Protein in Latex, Natural Rubber, and Elastomeric Products Using the Modified. RRIM (MS P) and the ASTM (D ) modified Lowry tests, were examined and their relationship studied. Total extractable proteins, EPRRiMt. ASTM De1, Standard Specification for Rubber Surgical Gloves ASTM D, Standard Test Method for the Analysis of Aqueous.

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Examples of test methods include, but are not limited to: Experimental s5712 and discussion Low-protein allergen analysis in latex compounded with fumed silica Compounding of fumed silica dispersion for dipped natural latex application resulted in tensile and tear enhancement as described before. The extracted protein is redissolved and quantified colorimetrically by the modified Lowry method using a protein standard.

Analytical information demonstrated that powdered latex gloves were found to contain high levels of protein between 1 and micrograms of protein per gram of rubber. Historical Version s d7512 view previous versions of standard.

ASTM D5712 – 15

It is suggested by R. Latex processed without silica, does have protein residues, see Table Ast. The fumed silica attaches itself to the rubber particle and substitutes the v5712.

Allergy and Clinical Immunology, 58, A non-silica rubber blank has micrograms of proteins per gram of rubber, see Table I. This test method covers an analytical test for determining the amount of total aqueous extractable protein associated with NR, latex, and elastomeric products.


It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. While donning and removing powdered gloves, the protein-coated particles become air borne dust and s5712 contaminate large volumes.


The silica also may serve as a thickening agent.

A general estimate is that only about 20 percent of the total protein evaluated by the ASTM test are harmful. This is in contradiction to what was proposed before where fumed silica attaches itself to the proteins on the surface of the latex particle, binding them there.

November 20, Page last updated: ASTM D might be misleading and can cause unfortunate conclusions. Two-sided coating is possible but expensive. At least two specific tests were developed to further evaluate allergenic protein levels. Natural proteins are the main reason for Type I latex irritation. D Specification for Rubber Surgical Gloves.

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Protein levels are below 1, the lowest level of detection, as clean as a vinyl film negative control. Latex protein analysis ASTM D, might not represent a true picture of the latex irritation problem.

There is a major distinction between powdered and powder-free gloves. A previous paper on cleaning latex films with s5712 silica5 presented protein results derived by ASTM D If you like to setup a quick demo, let us know at support madcad. The extracted protein is redissolved and quantified colorimetrically by the modified Lowry method using a protein standard.

Enter your personal account email address to request a password reset: Many gloves ast less proteins today, but protein cleaning is an important ongoing issue.

The proteins probably would stay adsorbed on the latex particles throughout the compounding process. Testing procedures required, please see astj full standard for complete details. Ast, powder-free films might have a different kind of a problem. As a consequence of the addition of ammonia for preserving the latex concentrate, the lipid materials are believed to hydrolyze slowly, releasing fatty acids.


Allergen units can range from 10, units per ml for an extract from raw latex film to 1 unit per ml extracted from vinyl glove film negative control or latex with undetectable level of allergenic proteins. For specific hazard statements, see Section 9. Here again, the presence of silica greatly diminished the level of latex allergenic proteins measured by the LEAP method.

A definitive procedure that produces a test result: Are you sure you want to empty the cart? Akron Rubber Development Laboratory, Inc.

Total and specific analysis of proteins in latex goods The total quantity of latex proteins in gloves is evaluated by extracting the rubber with an aqueous solution and measuring according to ASTM D Washing d512 minimal and since the latex is clean, there is no need for costly off line post treatments. The most common mechanism is direct contact. Land Use and Development. The astn then easily are removed. It was found that the addition of fumed silica to latex compounds helps in manufacturing of cleaner latex goods with lower protein d571.

Test Method A definitive procedure that produces a test result: FORMAT Single User Subscription Site License Subscription BOOKS All Books PACKAGES All Wstm EDITION to PUBLISHER.